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Seattle Pacific Carbohydrate Metabolism in Thermophilic Archaea Questions

Seattle Pacific Carbohydrate Metabolism in Thermophilic Archaea Questions

Question Description

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Carbohydrate metabolism in thermophilic archaea is different than that of eubacteria and mammals. For example, in the species Thermoproteus tenax the conversion of F6P to F-1,6-BP is not catalyzed by PFK-1. Rather, this reaction is catalyzed by a pyrophosphate-dependent phosphofructokinase (PPi-PFK). The reaction is:

Unlike mammalian PFK-1, the PPi-PFK reaction is readily reversible under physiological conditions.

a.In terms of the reactants and products, why is PPi-PFK reversible but PFK-1 irreversible? Be sure to reference ?G‡ and ?Grxn in your answer.

Is the PPi-PFK catalyzed-reaction near-equilibrium or far-from equilibrium? How do you know?

c.Sketch reaction coordinate diagrams for mammalian PFK-1 and PPi-PFK

d.Based on this information, would you expect PPi-PFK to be the flux-controlling enzyme for glycolysis in T. tenax? Why or why not?

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